![]() To use the SDM-Assist software you will need: Provides brief tips for primer design, logs the sequence of events as readable text in the display window.Exports all suggested primer sequences along with detailed information on them into an excel or text file.Allows user to customize choice of available restriction enzymes to SDM-Assist for inserting silent restriction sites in the primers.Scores suggested primer pairs on factors such as Tm, GC content, 5 prime and 3 prime-stability and secondary structure. ![]() Allows the user to generate and choose primers for SDM that contain a unique restriction site identity allowing for highly efficient identification of ‘mutated clones’ by a simple restriction digest. ![]() This is the Github repository for SDM-Assist software which is listed in the research paper " SDM-assist software to design site-directed mutagenesis primers introducing “silent” restriction sites. ![]()
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